Fig. 3
From: Metformin attenuates LPS-induced neuronal injury and cognitive impairments by blocking NF-κB pathway
LPS treatment results in synaptic dysfunction in rat primary hippocampal neurons. Western blotting (A) from homogenized primary hippocampal neurons showed the levels of synaptic protein detected by immunoblotting (B), n = 3. C–E Rat primary hippocampal neurons (DIV7) were incubated with LPS (1 μg/ml) for 6 h, and the dendritic morphology of hippocampal primary neurons following treatment with LPS was examined using anti-MAP2 antibody (C). The dendritic arborization complexity was tested by sholl analysis (D), and the total dendritic length was measured (E), n = 10. p value significance is calculated from a T-test, and data are presented as mean ± SEM. *p < 0.05, **p < 0.01 vs control group