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Table 1 Chronic unpredictable mild stress procedure

From: Electrolytic lesions of the bilateral ventrolateral orbital cortex not only directly reduce depression-like behavior but also decreased desperate behavior induced by chronic unpredicted mild stress in rats

  Monday Tuesday Wednesday Thursday Friday Saturday Sunday
First week 08:00 tilted cage 07:00 illumination 10:00–11:00 empty bottle 07:00 reversal illumination 10:00 tail clamp 10:00–11:00 SPT 10:00 flat cage
21:00 flat cage 15:00 deprive water 11:00 full bottle 13:00 monoculture 14:00 deprive water 11:00 provide water wet cage
illumination   18:00 two in a cage 13:00–19:00 stroboscopic lighting   15:00 tilted cage  
Second week 08:00 empty cage 08:00 restraint (2 h) 07:00 13:00 monoculture 14:00 deprive water 10:00–11:00 SPT 12:00 empty cage
Reversal illumination 11:00 provide water
18:00 clear bedding 17:00 deprive food 13:00–19:00 stroboscopic lighting 13:00 monoculture 17:00 wet cage
17:00 provide food
Third week 08:00 clear bedding 10:00–19:00 two in a cage 19:00 provide water 08:00 flat cage 08:00 restraint (2 h) 10:00–11:00 SPT 08:00 replace bedding
10:00 tail clamp 19:00 deprive water 19:00 tilted cage reversal illumination 14:00 deprive water 11:00 provide water 17:00 deprive food
18:00 illumination    13:00–19:00 stroboscopic lighting   15:00 wet cage  
Fourth week 10:00 provide food 10:00–11:00 empty bottle 08:00 restraint (2 h) 07:00 reversal illumination 13:00 flat cage 10:00–11:00 SPT  
11:00 provide water 11:00 replace bedding
10:00 provide food 19:00 illumination 08:00 restraint (2 h) 11:00–19:00 stroboscopic lighting 13:00 flat cage 10:00–11:00 SPT