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Table 1 Chronic unpredictable mild stress procedure

From: Electrolytic lesions of the bilateral ventrolateral orbital cortex not only directly reduce depression-like behavior but also decreased desperate behavior induced by chronic unpredicted mild stress in rats

 

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

First week

08:00 tilted cage

07:00 illumination

10:00–11:00 empty bottle

07:00 reversal illumination

10:00 tail clamp

10:00–11:00 SPT

10:00 flat cage

21:00 flat cage

15:00 deprive water

11:00 full bottle

13:00 monoculture

14:00 deprive water

11:00 provide water

wet cage

illumination

 

18:00 two in a cage

13:00–19:00 stroboscopic lighting

 

15:00 tilted cage

 

Second week

08:00 empty cage

08:00 restraint (2 h)

07:00

13:00 monoculture

14:00 deprive water

10:00–11:00 SPT

12:00 empty cage

Reversal illumination

11:00 provide water

18:00 clear bedding

17:00 deprive food

13:00–19:00 stroboscopic lighting

13:00 monoculture

17:00 wet cage

17:00 provide food

Third week

08:00 clear bedding

10:00–19:00 two in a cage

19:00 provide water

08:00 flat cage

08:00 restraint (2 h)

10:00–11:00 SPT

08:00 replace bedding

10:00 tail clamp

19:00 deprive water

19:00 tilted cage

reversal illumination

14:00 deprive water

11:00 provide water

17:00 deprive food

18:00 illumination

  

13:00–19:00 stroboscopic lighting

 

15:00 wet cage

 

Fourth week

10:00 provide food

10:00–11:00 empty bottle

08:00 restraint (2 h)

07:00 reversal illumination

13:00 flat cage

10:00–11:00 SPT

 

11:00 provide water

11:00 replace bedding

10:00 provide food

19:00 illumination

08:00 restraint (2 h)

11:00–19:00 stroboscopic lighting

13:00 flat cage

10:00–11:00 SPT