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Fig. 5 | BMC Neuroscience

Fig. 5

From: Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model

Fig. 5

Fluorescence-activated cell sorting (FACS) analysis of the apoptosis of dopaminergic SH-SY5Y cells and cytotoxicity assay analysis according to various siTRDN concentrations. A Annexin V-FITC (horizontal axis) and propidium iodide (vertical axis) staining results are shown in the dot plots. The FACS dot plots in the negative control (NC 100 nM siRNA) and 10-, 25-, 50-, and 100-nM siTRDN treatment groups. The H1-LR zone is analyzed as an apoptosis zone. B Percent of plots in the H1-LR zone are shown in a bar graph, indicating apoptosis. As the treatment concentration of siTRDN increases, apoptosis also increases (*P < 0.05). C Cytotoxicity assay analysis to show the same cell viability as in the B graph groups. Cell viability decreased from 25 nM of siTRDN significantly (*P < 0.05, **P < 0.005, ***P < 0.0005)

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