Table 1 Oligonucleotide primer sequences for quantitative reverse transcriptase polymerase chain reaction
Gene | Primer sequence (forward) | Primer sequence (reverse) | Annealing temperature (°C) | Product size (bp) | RefSeq Number |
|---|---|---|---|---|---|
TNFα | 5'- CGTCAGCCGATTTGCCATTT -3' | 5'- TCCCTCAGGGGTGTCCTTAG -3' | 60 | 88 | NM_012675.3 |
IL6 | 5'- AGAGACTTCCAGCCAGTTGC -3' | 5'- TGAAGTCTCCTCTCCGGACT -3' | 60 | 88 | NM_012589.2 |
IL1β | 5'- CACCTTCTTTTCCTTCATCTTTG -3' | 5'- GTCGTTGCTTGTCTCTCCTTGTA -3' | 60 | 241 | NM_031512.2 |
NFκB | 5'- TGTCTGCACCTGTTCCAAAGA -3' | 5'- TGCCAGGTCTGTGAACACTC-3' | 60 | 143 | NM_199267.2 |
RAGE | 5'- GGAAGGACTGAAGCTTGGAAGG -3' | 5'- TCCGATAGCTGGAAGGAGGAGT -3' | 60 | 102 | NM_053336.2 |
GAPDH | 5'- ATTGTTGCCATCAACGACCC -3' | 5'- TGACTGTGCCGTTGAACTTG -3' | 60 | 94 | NM_017008.4 |