Fig. 6
From: N/OFQ modulates orofacial pain induced by tooth movement through CGRP-dependent pathways
The effects of PNOC overexpression lentivirus vector on PNOC and N/OFQ expression. The rats were respectively administered with PNOC overexpression lentivirus (PNOC-lenti-OE), control lentivirus (ctrl-lenti) and normal saline (NS) one week prior to 40-g force application (n = 15 for each group, n = 3 for each group each day). a Temporal changes of PNOC mRNA expression level in trigeminal ganglia of rats in PNOC lentivirus group and control lentivirus group. One-way ANOVA with Bonferroni post hoc test was employed to analyze the effects of control lentivirus and PNOC overexpression lentivirus on PNOC mRNA expression. (*p < 0.05, **p < 0.01, ctrl-lenti group vs. PNOC-lenti-OE group). b Temporal changes of N/OFQ expression in trigeminal ganglia of rats in NS group, control lentivirus group and PNOC lentivirus group. One-way ANOVA with Bonferroni post hoc test was employed to analyze the effects of normal saline, control lentivirus and PNOC overexpression lentivirus on N/OFQ expression. (*p < 0.05, PNOC-lenti-OE group vs. NS group, PNOC-lenti-OE group vs. ctrl-lenti group). c Western blot analysis of N/OFQ expression in trigeminal ganglion of rats in NS group, control lentivirus group and PNOC lentivirus group using WES (NS normal saline, CV control lentivirus, V PNOC lentivirus). The images were cropped using Adobe Photoshop software, and full-length blots/gels are presented in Additional file 3: Figure S3