Fig. 5

Construction of PNOC overexpression lentivirus vector. a The map of GV320 vector. b Successful transduction of lentiviral vector into 293 T cells as shown by fluorescence microscopy that most cells were marked by enhanced red fluorescence protein (Cherry). The image was acquired at the resolution of 300dpi. c Western blot analysis of 293 T cells transfected with positive reference (SURVIVIN-3FLAG-GFP, 48KD) [1], not transfected [2], and transfected with PNOC lentiviral vectors (N/OFQ-3FLAG, 23KD) [3]. d Immunofluorescence image of TG in rat stained with cherry, which indicates successful transfection with PNOC lentivirus. The image was acquired at the resolution of 300dpi