Fig. 4
Hippocampal NeuN and Iba-1 expressing cells per area. In the top there is a representative micrography (×4) of the evaluated hippocampal regions (scale bar 200 µm) with the regions evaluated for NeuN and Iba-1. a CA1 NeuN (F[3, 20] = 0.2144, p = 0.8852) and b Iba-1 (F[3, 20] = 5.4, p = 0.0069) positive cells per area. c CA3 NeuN (F[3, 20] = 0.1392, p = 0.9354) and d Iba-1 (F[3, 20] = 4.08, p = 0.02) expressing cells per area. e Hippocampal hilus NeuN (F[3, 20] = 0.2.338, p = 0.1042) and f Iba-1 (F[3, 20] = 1.553, p = 0.2319) expressing cells per area. g Dentated Gyrus (DG) NeuN (F[3, 20] = 0.05, p = 0.985) and h Iba-1 (F[3, 20] = 5.785, p = 0.0051) expressing cells per area for the vehicles (VEH), adiponectin (APN), Amyloid-β (Aβ) and APN–Aβ injected rats. Data are presented as mean + SEM and evaluated using a One-way ANOVA with a post-hoc Tukey test. Asterisks represent significant differences (*p < 0.05, **p < 0.01). Higher magnifications of each region for both antibody stainings are presented in Additional file 1: Fig. S1