Fig. 2

Immunohistological determination of NeuN and Iba-1 expression in the olfactory bulb. a Representative micrographies of the olfactory bulb showing the evaluated areas for NeuN and Iba-1, scale bar 250 µm; the arrows denote the region where glomeruli are disarranged. b Representative micrographies of NeuN (top) and Iba-1 (bottom) of the granular cell layer (GCL) with examples of the random squares (ROIs) quantified, scale bar 50 µm. c Number of NeuN expressing cells in the GCL for Vehicles (VEH), adiponectin (APN), Amyloid-β (Aβ and APN–Aβ injected rats (F_{[3, 20]}  = 0.1, p < 0.94). d Number of Iba-1 positive cells per area of the GCL of the same groups (F_{[3, 20]} = 0.6336, p < 0.602). e Representative micrographies of the glomeruli with examples of the random squares (ROIs) quantified, scale bar 50 µm. f Number of NeuN expressing cells in the glomeruli per area of the same groups (F_{[3, 68]} = 15.93, p < 0.0001). g Circularity index of the glomeruli of the same groups (F_{[3, 312]} = 79.54, p < 0.0001). h Number of Iba-1 positive cells per area (F_{[3, 20]} = 0.7029, p < 0.5614. Data are presented as SEM and evaluated using a One-way ANOVA with a post-hoc Tukey test