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Fig. 3 | BMC Neuroscience

Fig. 3

From: Docosahexaenoic fatty acid reduces the pro‐inflammatory response induced by IL-1β in astrocytes through inhibition of NF-κB and AP-1 transcription factor activation

Fig. 3

The level of COX-2 and iNOS proteins (a, b) and TNF-α and IL-6 (C and D) in astrocytes. Proinflammatory enzyme expression was determined in whole cell lysate after incubation of cells with 30 µM DHA, followed by IL-1β (10 ng/ml) treatment. Full-length blots are presented in Additional file 2: Fig. S2. The concentration of cytokines was measured twice in triplicate in media from astrocytes incubated with various concentrations of DHA and treated with IL-1β using an ELISA-based method. The data are given as means ± SEM. **p < 0.01, ***p < 0.001 compared to control, #p < 0.05, ##p < 0.01, ###p < 0.001 compared to IL-1β-treated cells

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