Fig. 1

Proximity ligation assay showing pre- and postsynaptic localization of ADAM10 and BACE1 in adult rat brain. Adult rat brain hippocampal sections were labelled with primary antibodies (anti-ADAM10 or anti-BACE1 made in rabbit and anti-synaptophysin or anti-PSD-95 made in mouse) followed by secondary oligonucleotide-linked probes. Each PLA signal (brown dot) denotes two proteins within 40 nm distance from each other; a ADAM10 and synaptophysin, b ADAM10 and PSD-95, c BACE1 and synaptophysin and d BACE1 and PSD-95. In the negative control samples only one primary antibody (anti-synaptophysin (e), anti-PSD-95 (f), anti-ADAM10 (g) or anti-BACE1 (h)) was used. Brain tissue and cell nuclei were visualized by a nuclear stain solution containing Mayer´s haematoxylin. Each experiment was performed five times (technical replicates) and representative images are shown. Scale bar 20 µm