Fig. 1

Representative images of hippocampal CA1 slices exposed to normoxic and OGD conditions from male and female animals (a). All cell nuclei are shown by Hoechst staining and cell death is shown in images stained with PI (a). Cell death was analysed for both the CA1 and dentate gyrus (DG) regions and was found to be significantly increased following exposure to OGD (b, *P < 0.001 vs. normoxic control). In addition, following OGD, the amount of cell death seen in male-derived slices was significantly increased compared to female animals in both CA1 and DG regions (# P < 0.001). Data are expressed as mean ± SEM and n = 8 independent wells. Scale bars represent 20 µm