Fig. 3

Chronic oxycodone treatment activates the Integrated Stress Response in rat blood. Western blot analysis of phosphorylated eIF2α in rat blood. Left panel, equal amount of total protein from blood of rats treated with oxycodone (O1, O2) or water (W) corresponding to samples presented in Fig. 2a. was analysed by western blot probed with anti-phospho eIF2α and total eIF2α. Right panel, graph representing mean value of phospho-eIF2α to total eIF2α ratio normalized to that in water samples set as one (± SEM; n = 4 water and n = 5 oxycodone (three sets of experiments that analyzed samples from 8 water and 13 oxycodone treated rats; p = 0.048). Statistical analysis of carbonyl content in oxycodone relative to water exposed cortex lysates was performed using Student’s t-test