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Fig. 1 | BMC Neuroscience

Fig. 1

From: Carbonyl-protein content increases in brain and blood of female rats after chronic oxycodone treatment

Fig. 1

Chronic oxycodone treatment increases protein carbonyl content in rat cortex. a BioVision carbonyl content assay of proteins from cortex lysates of rats treated with water (W) or oxycodone (O1, O2). Left panel, assay was performed according to a manufacture protocol using four sets of animal groups that represented four different litters, labeled as Exp. #. Data expressed as nanomol of carbonyl groups per milligram of protein using BSA as standard. Right panel, graph representing mean value of carbonyl content in cortex lysates normalized to the corresponding water samples set as one for each experiment (± SEM; n = 4 (four BioVision experiments that analyzed samples from 10 water and 20 oxycodone treated rats; p = 0.012). Statistical analysis of carbonyl content in oxycodone relative to water exposed cortex lysates was performed using Student’s t-test. b Immunofluorescent analysis of carbonylated proteins in cortex of rats treated with water (W) or oxycodone (O). Scale bar denotes 50 µm for the top striatum images and 5 µm for the middle striatum and lower corpus callosum images

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