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Table 1 Relative gene expression in the adrenal (Mc2R and Hsd1) and pituitary (Crfr1) in WT and Per2−/− mice measured at lights on (1300 h)

From:  Knockout of the circadian gene, Per2, disrupts corticosterone secretion and results in depressive‐like behaviors and deficits in startle responses

Gene Accession # Primer sequence WT Per2−/−
Adrenal
 MCR2 NM_0010839 (F) 5’-ACACCAATGACACCGCAAGA-3’
(R) 5’-ACAGACTGCCCAACATGTCA-3’
1.0 ± 0.2 1.3 ± 0.4
 HSD1 NM_008288.2 (F) 5’-AGCCGCACTTATCTGAAGCC-3’
(R) 5’-TTCCCTGGAGCATTTCTGGTC-3’
1.0 ± 0.3 0.7 ± 0.3
Pituitary
 CRFR1 NM_007762 (F) 5’-CAAAGTGCACTACCACATTGCCGT-3’
(R) 5’-TGAAAGCCGAGATGAGGTTCCAGT-3’
1.0 ± 0.2 0.7 ± 0.3
  1. There were no differences in gene expression between WT and Per2−/− tissues (p > 0.05; n = 5–8 animals per genotype). Primer information is also provided