Knockout of the circadian gene, Per2, disrupts corticosterone secretion and results in depressive‐like behaviors and deficits in startle responses

Table 1 Relative gene expression in the adrenal (Mc2R and Hsd1) and pituitary (Crfr1) in WT and Per2−/− mice measured at lights on (1300 h)

Gene	Accession #	Primer sequence	WT	Per2−/−
Adrenal
MCR2	NM_0010839	(F) 5’-ACACCAATGACACCGCAAGA-3’ (R) 5’-ACAGACTGCCCAACATGTCA-3’	1.0 ± 0.2	1.3 ± 0.4
HSD1	NM_008288.2	(F) 5’-AGCCGCACTTATCTGAAGCC-3’ (R) 5’-TTCCCTGGAGCATTTCTGGTC-3’	1.0 ± 0.3	0.7 ± 0.3
Pituitary
CRFR1	NM_007762	(F) 5’-CAAAGTGCACTACCACATTGCCGT-3’ (R) 5’-TGAAAGCCGAGATGAGGTTCCAGT-3’	1.0 ± 0.2	0.7 ± 0.3

There were no differences in gene expression between WT and Per2−/− tissues (p > 0.05; n = 5–8 animals per genotype). Primer information is also provided

ISSN: 1471-2202