Fig. 4

Enriched environment ameliorates defects in adult neurogenesis due to Tcf4 haploinsufficiency. a Representative images and quantification of the number of proliferative cells, NESTIN + (aNSCs) and the activation of aNSCs in the DG after five weeks of EE. The number of proliferative cells, aNSCs and the activation ratio of aNSC is unaltered. Scale bar, 50 µm; n = 5. b Representative images and quantification of BrdU incorporating cells in the DG four weeks after last BrdU injection and after five weeks of EE. Tcf4Het EE mice have an increased number of BrdU + cells in DG. Scale bar, 100 µm; n = 5. c Representative images and quantification of the number of BrdU + single, BrdU + DCX + PROX1 + triple and BrdU + PROX1 + double positive cells four weeks after last BrdU injection and after five weeks of EE. The number of immature neurons (BrdU + DCX + PROX1 + triple positive) is decreased in Tcf4Het EE animals, whereas the number of mature neurons (BrdU + PROX1 + positive) is increased. Scale bar, 50 µm; n = 5. Data are presented as mean ± SEM