Table 2 Description of primary antisera used

Aromatase

Novus Biologicals LLC, Centennial CO, US

NB100-1596

AB_10000919

WB; 1:2000 (0.5 μL/mL)

Human Aromatase protein (between residues 400-502). [UniProt# P11511]

Giles et al., Breast Cancer Res. 2018; 20(1): 50

Neuronal Nitric oxide synthase (nNOS)

Novus Biologicals

NBP1-39681

AB_2282822

WB; 1:1500 (0.75 μL/mL)

Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS)

Mahmood et al., Mol. Cell. Neurosci. 2019; 95: 51-58

Glutamate Decarboxylase 65/67 (GAD)

Millipore Sigma, Burlington, MA, US

ABN904

Not available

WB; 1:10000 (0.1 μL/mL)

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human glutamate decarboxylase 2 (GAD2).

Ibrahim, et al., Brain Res. 2019; 1711: 48-57

Glycogen Phosphorylase-Brain Type (GPbb)

Novus Biologicals

NBP1-32799

AB_2253353

WB; 1:2000 (0.5 μL/mL)

Recombinant protein encompassing a sequence within the C-terminus region of human GPBB. The exact sequence is proprietary

Ali MH et al.; Neuroscience 2019; 409: 253-260

Glycogen Phosphorylase-Muscle Type (GPmm)

Novus Biologicals

NBP2-16689

Not available

WB; 1:2000 (0.5 μL/mL)

Recombinant protein encompassing a sequence within the center region of human PYGM. The exact sequence is proprietary

Ali MH et al.; Neuroscience 2019; 409: 253-260

Glycogen Synthase (GS)

Cell Signaling

Technology LLC, Danvers CT, US

3893S

AB_2279563

WB; 1:2000 (0.5 μL/mL)

Antibody detects total muscle glycogen synthase protein

Ibrahim et al., Brain Res. 2019; 1711: 48-57

Glutaminase (GLT)

Novus Biologicals

NBP1-89766

AB_11013964

WB; 1:1500 (0.75 μL/mL

DRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGL

Uddin et al., Brain Res. 2019; 1720: 146311

Malic Enzyme 1 (ME1)

Novus Biologicals

NBP1-32398

AB_2143825

WB; 1:2000 (0.5 μL/mL)

Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary

Uddin et al., Brain Res. 2019; 1720: 146311