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Table 1 Experimental design

From: Sex-dimorphic neuroestradiol regulation of ventromedial hypothalamic nucleus glucoregulatory transmitter and glycogen metabolism enzyme protein expression in the rat

 

Icva Pretreatment

Subcutaneous injection

Group identifier

Day 5-Day

Day 11

Treatment groups

Male

 Vb

Vc

V/V-M

n = 6

 V

INSd

V/INS-M

n = 6

 Lze

V

Lz/V-M

n = 6

 Lz

INS

Lz/INS-M

n = 6

Female

 V

V

V/V-F

n = 6

 V

INS

V/INS-F

n = 6

 Lz

V

Lz/V-F

n = 6

 Lz

INS

Lz/INS-F

n = 6

  1. a Left lateral ventricle
  2. b Artificial cerebrospinal fluid (30%)/dimethyl sulfoxide (70%)
  3. c Sterile diluent; 100 μL/100 g bw
  4. d 10.0 U neutral protamine Hagedorn insulin/kg bw
  5. e 1.67 μg/μL dosage, 0.5 μL/h infusion rate, 7 day duration of pretreatment