Fig. 4

Effects of MgT and MgSO₄ on neurons after normoxia (left) or hypoxia (right) in the presence or absence of MgT or MgSO₄. MgT: magnesium l-threonate. The cells were treated with PBS (control), MgSO₄ (1 or 10 mM) or MgT (1 or 10 mM) for 1 h. After the culture media were exchanged, the cells were incubated for 2 h in a CO₂ incubator or a hypoxic chamber. The cellular experiments were repeated three times (each individual experiment contained 6 technical replicated, totaling 180 wells). Data are shown as mean ± SEM. ^†p < 0.05 compared to the PBS group, ^‡p < 0.05 compared to the PBS + HYP group. PBS: phosphate-buffered saline; MgT, magnesium l-threonate; HYP, hypoxia