Fig. 2

The hcfc1a^co60/+ allele increases the number of Sox2⁺ cells in the developing brain. a Quantitative real time PCR analyzing the relative expression of sox2 and pax6 in wildtype siblings (Sibling) and the hcfc1a^co60/+ allele (Co60/+). N = 7 larvae per group/biological replicate *p < 0.05. b–d Quantification of the number of Sox2⁺ cells at 1 day post fertilization (DPF) (b), 2 DPF (c), and 5 DPF (d) in the early forebrain (EF), forebrain (FB), midbrain (MB), and hindbrain (HB). *p = 0.006828, **p = 1.13873E−05, ***p = 0.000107, ^#p = 0.010168, ^##p = 4.55E−06, ^###p = 1.97E−07, and ^§p = 0.005476. In b total number of animals is Sibling (N = 6) and hcfc1a^co60/+ (N = 6), c total animals is Sibling (N = 7) and hcfc1a^co60/+ (N = 7), and d total animals is Sibling (N = 4) and hcfc1a^co60/+ (N = 4). All error bars represent standard error of the mean