Fig. 4

The expression of COX5A in OGD neurons. a The bright field of neurons in normal and OGD groups. Scale bar = 100 μm. b Quantitative analysis of relative mRNA expression of COX5A in neuron. N = 6/group. c Immunofluorescent staining of COX5A in normal and OGD groups. Red staining represents the COX5A positive cells, and the nucleus is stained by blue, n = 3/group. Scale bar = 200 μm. d The COX5A positive cells per field were calculated. N = 6/group. OGD oxygen–glucose–deprivation, COX5A cytochrome c oxidase subunit 5a, qRT-PCR quantitative real-time polymerase chain reaction. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01