Fig. 3
From: The Bdnf and Npas4 genes are targets of HDAC3-mediated transcriptional repression
PCR validation of ChIP-Seq data. ChIP was used to validate the ChIP-Seq results. For this, ChIP was conducted on genes that were positive for HDAC3 occupancy based on ChIP-Seq (Npas4 and Bdnf) and three genes known to have neuroprotective effects but were not among the positive hits in the ChIP-Seq (cFos, FoxP1, Stat3). PCR was conducted following immunoprecipitation of sheared chromatin using primers to the promoter regions of these genes (see Table 1 for primer sequences)