Fig. 5
From: Inhibition of autophagy triggers melatonin-induced apoptosis in glioblastoma cells
Melatonin induced autophagy by enhancing autophagosome formation. U87MG (A-B) and A172 (C-D) cells were treated with PBS (control), rapamycin (Rap 200 nM), melatonin (MLT 1 mM), or agomelatine (Ago 2 μg/ml) for 24 h in the presence or absence of 400 nM bafilomycin A1 (added 4 h before cell harvesting). The endogenous LC3-II levels were detected by Western blotting with anti-LC3 antibodies and were quantified by a densitometric analysis relative to GAPDH (**p < 0.01, compared to the control for each group, n = 3)