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Fig. 3 | BMC Neuroscience

Fig. 3

From: Inhibition of autophagy triggers melatonin-induced apoptosis in glioblastoma cells

Fig. 3

The time-dependent effects of melatonin on the LC3-II and Beclin 1 expression in U87MG (a, b) and A172 (c, d) cells. The cells were treated with 1 mM melatonin for 4, 12, 24 or 36 h. PBS was used as a negative control, and rapamycin (Rap 200 nM) served as a positive control. The levels of LC3-II and Beclin-1 expression were determined by a Western blot analysis. The protein bands for each regimen were quantified by densitometry, and their differences are presented in the graph. The values represent the mean ± S.E.M. for three separate determinations. Statistical significance * at P < 0.05 or ** at P < 0.01 compared with untreated controls

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