Fig. 1
From: Inhibition of autophagy triggers melatonin-induced apoptosis in glioblastoma cells
Melatonin induced LC3 aggregation and conversion. a GFP-LC3 aggregation and conversion in U87MG cells stably expressing an exogenous fusion protein, green fluorescent protein (GFP) and microtubule-associated light chain 3 (LC3) (U87-GFP-LC3), after treatment with DMSO (control), rapamycin (RAP 200 nM), melatonin (MEL 1 mM) or agomelatine (AGO 2 μM) for 24 h (×40); b U87MG cells and c A172 cells treated with DMSO (control), rapamycin (RAP 200 nM), melatonin (MEL 1 mM), agomelatine (AGO 2 μM) and luzindole (LUZ 5 μM/L) for 24 h were fixed and analyzed for endogenous LC3 dots by immunofluorescence using anti-LC3 antibodies (green), DAPI (blue) staining shows the nuclei (×40). d Stable U87-GFP-LC3 cell lines and GFP in vector control. U87MG cells stably expressing an exogenous fusion protein, green fluorescent protein (GFP) and microtubule-associated light chain 3 (LC3) (U87-GFP-LC3) (×40)