Fig. 8
From: Localization of phosphotyrosine adaptor protein ShcD/SHC4 in the adult rat central nervous system
Immunolocalization of ShcD in rat neurons and neural precursors. A Dual immunoperoxidase (DAB) and immunofluorescence histochemistry of ShcD and neuronal marker NeuN within the cortex indicates ShcD expression in neurons (see zoom). B Immunofluorescence of the subventricular zone (SVZ) on near adjacent coronal sections shows overlapping expression patterns of ShcD and neural precursor marker Nestin, with ShcD staining most prominent in cells lining the lateral ventricle. Hoechst staining denotes nuclei. Arrows indicate nestin expressing cells lining the lateral ventricle. C Dual immunofluorescence labeling of the dentate gyrus subgranular layer shows co-staining of ShcD and Nestin (see arrows and zoom). Scale bars: A, C = 100 μm, B = 50 μm