Fig. 2

Action potentials for a diazepam (DZP) and b ethanol (EtOH) relative to control conditions. Both diazepam (blue, left) and ethanol (red, right) decreased the number of action potentials in a concentration-dependent manner. a 30 µM diazepam had a strong effect on the spiking rate compared versus control conditions. Diazepam caused a decrease in the number of action potentials as indicated by Hedge’s g (g = 1.20 [0.70 2.59]) that was not significant after Bonferroni correction (p = 0.031, uncorrected). The decrease in spike rate from 15 µM to 30 µM diazepam was significant and strong (p = 0.0156; g = 1.20 [0.72 2.57]). b When compared to control conditions, 30 mM ethanol did not show a significant reduction of spike rate (p = 0.054, g = 0.43 [− 0.08 1.16]), but 60 mM significantly reduced the spiking rate (p = 0.003; g = 0.69 [0.31 1.26]). The change from 30 mM to 60 mM ethanol was weak and not significant after Bonferroni correction (p = 0.042, uncorrected; g = 0.22 [− 0.11 0.51]). *p < 0.05 Bonferroni corrected; ^#p < 0.05 uncorrected