Fig. 4

CS neurons projecting to dorsal and ventral horns segregates into distinct functional ensembles. a Representative traces of calcium activity for 8 simultaneously imaged CS neurons projecting to DH (green) and IVZ (red). b (graphic representation of the correlation of calcium ongoing fluctuations between all the simultaneously imaged neurons projecting to the DH (green) and IVZ (red) obtained in one experiment. Circles represents the imaged neurons and connecting lines represents the positive significant Pearson correlation between elements in the network. The asterisks mark the neurons of the traces in a. The shaded areas group the neurons according to the correlation strength into modules based on modularity algorithm proposed by Blondel et al. [28]. Interestingly, in all the experiments (n = 8) two modules has been defined by the algorithm and were named arbitrarily as Module 1 and 2. The graphic representation of the functional networks was performed with the open access software Gephi (v 0.9.2). c proportion of neurons projecting to DH and IVZ assigned to the Module 1 computed for all the experiments. d The same as c but for module 2