Fig. 3

Propofol post-conditioning enhanced the combination of GluR2 and PI3K. A The western blotting assay of PI3K–GluR2 complex. The tissue from ischemic hippocampus was incubated with antibody of GluR2. The complexes were collected on protein A/G beads. Anti-PI3K antibody was used for investigating the expression of PI3K–GluR2 complex in the immunoprecipitation at 85 kDa. Anti-GluR2 antibody was to detect the concentration of total GluR2 which reflected at 100 kDa. S: sham-operated group; IR: P: propofol Post-cond group; L + S: sham-operated group; L + IR: LY294002 + ischemia–reperfusion injury group; L + P: LY294002 + propofol Post-cond group. B The statistical result of optical density was gathered and compared with Shame Group to get the results of complex. compared S Group, other groups at any time point had more expression of PI3K–GluR2 complex; while in Group P the complex containing was higher compared with Group IR (P < 0.0001, P < 0.0001, and P = 0.001 at each time-point).; applied LY294002 depressed the trend which made by propofol (P = 0.015, P = 0.011, and P = 0.023 at each time-point). n = 6 for each group.*P < 0.05 vs Group IR; **P < 0.01 vs Group IR; ^#P < 0.05 vs Group P. C The expression of total GluR2. There were no significant differences in each group at each time point