Fig. 3

Representative images of S-opsin⁺ cones and M/L-opsin⁺ cones in retinal transverse sections of Rd1 mice at postnatal day (P) 14, P21 and P60. Double labeling immunofluorescence in the superior (a–c) and inferior retina (d–f) was performed using antibodies directed against S-opsin (red) and M/L-opsin (green). Dual cones express both S-opsin and M/L-opsin and appear yellow (arrows). Scale bar: 20 mm. IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer