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Fig. 6 | BMC Neuroscience

Fig. 6

From: Post-surgical inhibition of phosphatidylinositol 3-kinase attenuates the plantar incision-induced postoperative pain behavior via spinal Akt activation in male mice

Fig. 6

Post-surgical inhibition of PI3K attenuated spinal Akt activation induced by plantar incision in male mice. PI3K inhibitor wortmannin (0.4 μg) or LY294002 (5 μg) or DMSO was intrathecally injected at 45 min after plantar incision. The expression of spinal pAkt was assayed at the 2-h time-point after plantar incision. Post-surgical inhibition of PI3K with both wortmannin (a) and LY294002 (b) attenuated spinal Akt activation induced by plantar incision. The representative bands (top) and the quantitative data (bottom) for the expression of spinal pAkt after plantar incision. The fold change for the density of each pAkt was normalized to total Akt for each sample. The fold change of pAkt level in Sham-DMSO group was set at 1 for quantifications. ##P < 0.01 compared with Sham-DMSO group; *P < 0.05, compared with Incision-DMSO group (one-way ANOVA, followed by Bonferroni’s multiple comparison test; n = 4 mice in each group). Data are presented as mean ± SEM

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BMC Neuroscience

ISSN: 1471-2202

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