Table 1 List of antibodies in the study for immunofluorescence or western blot analysis

Monoclonal mouse anti-MBP (SMI-99)

Millipore

Cat#NE1019, RRID:AB_2140491

Purified human myelin basic protein with amino acids 131–136

1:200 (IF)

Monoclonal mouse anti-MBP (SMI-94)

Millipore

Cat#NE1018, RRID:AB_2140494

Purified human myelin basic protein with amino acids 70–89

1:1000 (WB)

Polyclonal rabbit anti-PLP

Abcam

Cat#ab28486, RRID:AB_776593

Synthetic peptide as amino acids 109–128 of mouse myelin PLP

1:1000 (WB)

Polyclonal rabbit anti-Iba1

Wako

Cat#019-19741, RRID:AB_839504

Purified by the antigen affinity chromatography from rabbit antisera

1:200 (IF)

Monoclonal rat anti-CD11b

BD Biosciences Cat#550282, RRID:AB_393577

Rat (DA) IgG2b, κ in mouse splenic cells

1:200 (IF)

Polyclonal rabbit anti-Olig2

Millipore

Cat#AB9610, RRID:AB_570666

Recombinant mouse Olig-2

1:200 (IF)

Polyclonal rabbit anti-GFAP

Millipore

Cat#AB5804, RRID:AB_2109645

Purified bovine GFAP

1:200 (IF)

Polyclonal goat anti-IL-33

R and D Systems Cat#AF3626, RRID:AB_884269

Escherichia coli-derived recombinant mouse IL-33

1:200 (IF)

Monoclonal mouse anti-IL-33 (Nessy-1)

Enzo Life Sciences

Cat#ALX-804-840/1, RRID:AB_11000255

Recombinant human IL-33 (aa 112-270) (Prod. No. ALX-522-098)

1:1000 (WB)

Monoclonal mouse anti-β-actin

Santa Cruz Biotechnology Cat#sc-81178, RRID:AB_2223230

Recombinant protein corresponding to a region near the C-terminus of β-Actin of human origin

1:1000 (WB)

Monoclonal mouse anti-GAPDH

Millipore

Cat#MAB374, RRID:AB_2107445

Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle

1:2000 (WB)