Fig. 3
Two-photon imaging of Ae. aegypti larvae. Example images of two-photon microscopy imaging of live Ae. aegypti larvae. Areas of interest included the optic lobe; OL, supra-esophageal ganglion; SuEG (upper DE, (a)), subesophageal ganglion; SOG (b), antennal nerve; AN and antennal lobe; AL. Cell bodies (labeled with a filled arrow), neuropil (labeled with an open arrow). (c). [A; B: L4 larvae with dorsal head cuticle removed. c L2 larva imaged through transparent cuticle]. d Approximate 3D reconstruction of larval brain regions based on a confocal scan of the dissected larval brain. Asterisks indicate the rough location of the antennal lobe. Additional labeled brain regions include a general schematic of the optic lobe (OL), deutocerebrum (DE), mushroom bodies (MB), suboesophageal ganglion (SOG), and oesophageous foramen (OF) [35], Thermo Scientific Amira Software)