Neuro-genetic plasticity of Caenorhabditis elegans behavioral thermal tolerance

BMC Neuroscience

Table 2 trpa-1 construct lines assayed for TTCs

Strain^a	Construct	Genetics	Explanation
RB1052	–	trpa-1(ok999)	trpa-1 knockout mutant
TQ1643	XuEx601	XuEx601[Pges-1::trpa-1::SL2::yfp + Punc-122::DsRed]	Gut promotor overexpression
TQ1648	XuEx606	XuEx606[Prgef-1::trpa-1::SL2::yfp + Punc-122::DsRed]	Neural promotor over expression
TQ2706	XuEx619 and XuEx735	trpa-1(ok999); lin-15; xuEx735[Pges-1::trpa-1::SL2::mcherry2 + Punc-122delta::yfp]; xuEx19[Plfe-2L::GCaMP1.3 + Plfe-2L::DsRed2b + lin-15(+)]	trpa-1 rescued with own genomic DNA promotor

^aXiao et al. (2013); We tested strain TQ2706 as a trpa-1 rescue using our own upstream genomic promotor. Lines with constructs XuEx601 and XuEx606 were backcrossed separately to strain RB1052 to create tissue-specific trpa-1 rescue lines, confirmed by PCR and fluorescence

ISSN: 1471-2202