Fig. 4

Comparison of wildtype and trpa-1 mutants over different rearing temperatures. a The sensitivity of the locomotion index TTC to rearing temperature observed for wildtype N2 C. elegans (dashed lines) is reduced for trpa-1 mutant animals (dark lines), which show similar TTCs regardless of rearing conditions. Error bars indicate ± SEM; 54 animals tested per treatment, ≥ 44 individuals included in calculations at each assay step. b Comparison of inflection point estimates for locomotion index TTC curves for wildtype N2, trpa-1 and ttx-1 mutants, and trpa-1 expression-rescue strains (see Table 2 and Additional file 1: Fig. S3). Similar inflection point values for different rearing conditions of a given genotype indicate low TTC plasticity; distinct values for different rearing conditions indicate high TTC plasticity. Genomic and gut expression-rescue constructs for trpa-1 also rescue TTC plasticity, unlike the neural expression-rescue construct. Values for wildtype N2 and trpa-1(ok999) correspond to TTCs shown in a. Error bars indicate 95% CI from a three-parameter logistic function fit to locomotion index (LI1) values for each strain and rearing temperature; 45–54 animals tested per treatment, ≥ 31 individuals included in calculations at each assay step