Fig. 7
From: Iron concentrations in neurons and glial cells with estimates on ferritin concentrations
Differential centrifugation protocol. Differential centrifugation protocol for fresh neocortex homogenate in 0.25 M saccharose solution to obtain the following subcellular fractions: nucleus fraction (blue), mitochondria fraction (green) and microsome fraction (red). The enrichment of the target particles is verified by transmission electron microscopy. The nucleus fraction contains cell nuclei (highlighted in blue, nucleolus in darker blue) as well as myelin fragments as seen at the very bottom of the picture. The mitochondria fraction is enriched in numerous mitochondria (highlighted in green), but also synaptosomes (highlighted in yellow), Golgi complexes (highlighted in light blue) and lysosomes (highlighted in red). The microsomal fraction contains microsomes (highlighted in red, summarizing vesicles, plasma membranes, ribosomes and endoplasmic reticulum)