Fig. 4
Sox10 is rapidly downregulated in Wnt1-Cre; Mib1fl/fl embryos during early neural crest cell migration. a Transverse sections over the forelimb region of wildtype and Wnt1-Cre; Mib1fl/fl embryos at E9.25 (20 somite pairs) co-immunostained for the migrating neural crest cell markers AP2α and Sox10. Inset images in lower panels show higher magnification of the boxed region in upper panels. Scale bar = 50 μm. b Transverse sections of embryos at E9.5 (23 somite pairs) co-immunostained for AP2α and Sox10. Scale bar = 50 μm. c Transverse sections at E9.75 (26 somite pairs) co-immunostained for Isl1 and Sox10. Scale bar = 50 μm. d Quantitation of AP2α-positive and Sox10-positive cells per migrating neural crest stream at E9.25. Number represents mean of n = 3 embryos, with over 20 individual sections counted per embryo. ns = not significant. e Quantitation of E9.5 embryos, n = 4 embryos. *p < 0.05. f Quantitation of E9.75 embryos, n = 3 embryos, nt neural tube, **p < 0.01