Fig. 8

Effect of CTX-B on mechanical and thermal allodynia in stressed rats with thermal injury. CTX-B administration for 1–8 days significantly attenuated stress-induced enhanced mechanical allodynia in thermal injured rats at all times of testing. Both S + I + Saline and S + I +CTX-B groups showed significant reduction in PWT when compared to their respective baseline threshold on behavioral testing days 7–14. However, the S + I + Sal treated group showed lower PWTs on post-injury days 7–14 compared to the S + I + CTX-B group. **** = P < 0.0001 indicates significant differences between S + I + Sal and S + I + CTX-B treated groups. ^# = P < 0.05, ^## = P < 0.01, ^### = P < 0.001 ^#### = P < 0.0001 indicates significant difference in comparison to their respective baseline threshold (a). In the contralateral paw, CTX-B-treated group showed increased withdrawal threshold compared to saline-treated group on post-injury day 14. *** = P < 0.001 indicates significant difference between S + I + Sal and S + I + CTX-B treated groups (b). No significant change in PWL was observed between S + I + Sal and S + I + CTX-B-treated groups throughout the testing period. However, both groups showed significant reduction in withdrawal latency when compared to respective baseline paw withdrawal latency. ^#### = P < 0.0001 indicates significant difference between S + I + Sal and their baseline latency; **** = P < 0.0001 and *** = P < 0.001 compared between S + I + CTX-B and baseline PWL (c). Contralateral PWL was comparable between S + I + Sal and S + I + CTX-B-treated groups (d). NS non-stress, S stress, Sal saline, I injury; CTX-B: cyclotraxin; n = 6/group. Data is represented as mean ± SEM