Fig. 3

CIS procedure exacerbates mechanical allodynia without effecting thermal allodynia in thermally injured rats. Thermal injury to ipsilateral paw produced time-dependent reduction in withdrawal threshold to mechanical stimulus in NS + I and S + I groups. However, S + I group showed enhanced mechanical allodynia. ^#### = P < 0.0001 indicates significant difference when compared to their respective baseline threshold. ** = P < 0.01 and **** = P < 0.0001 indicates significant differences between NS + I and S + I groups (a). At 10, 12, and 14 days post-thermal injury, the uninjured contralateral paw of rats from S + I group showed significant decrease in PWT compared to the NS + I group. * = P < 0.05 and ** = P < 0.01 indicates significant difference between NS + I and S + I groups (b). Thermal injured ipsilateral paw from NS + I and S + I groups showed significant decrease in PWL compared to their respective baseline values. The changes were observed throughout the testing period. No significant change in PWL between NS + I and S + I groups occurred. ^#### = P < 0.0001 indicates significant difference compared to baseline PWL (c). In contralateral paw no significant changes in PWL between NS + I and S + I groups were observed (d). NS non-stress; S stress; I Injury. n = 9/group. Data is represented as mean ± SEM