Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury

Table 4 Animal experimental treatment group assignments

Group	N	Label	Condition	Level	Survival post-labeling	Survival post injury
1	10	FG	TXN	T₁₀	10 days	3 days
2	10*	FG	TXN	T₂	10 days	3 days
3	10*	FG	Control	No injury	10 days	–

Animal experimental treatment groups. Female Fischer 344 rats were divided among 3 groups. Using the indicated tracer, Fluorogold (FG) PS neurons were labeled via bilateral injections of FG into the lumbosacral enlargement prior to axotomy. The animals were allowed to recover for 1 week, allowing the FG adequate time to retrogradely label PS neurons. Following 1-week tracer transport time, animal is groups 2 and 3 then received a complete spinal transection (TXN) at the indicated spinal level, and were then allowed to recover for 3-days, at which point the spinal tissue was harvested for laser capture microdissection and gene expression analysis
*Three animals in the T₂ SCI group and 1 in the uninjured control group were euthanized prior to the planned date due to health complications

ISSN: 1471-2202