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Fig. 5 | BMC Neuroscience

Fig. 5

From: Rapamycin treatment increases hippocampal cell viability in an mTOR-independent manner during exposure to hypoxia mimetic, cobalt chloride

Fig. 5

Rapamycin treatment favors autophagy during CoCl2 exposure. a Rapamycin treatment restores expression of autophagy marker, Beclin1. Cells were treated and analyzed as in Fig. 5a via Western blot. Left is the average relative protein expression of Beclin1 normalized to β-actin protein expression. Right is a representative image of at least three separate blots showing Beclin1 protein expression. ###p < 0.001 versus control; **p < 0.05 versus CoCl2 alone. b Ratio of LC3-II/LC3-I is elevated during CoCl2 exposure and further increased with rapamycin addition. HT22 cells were treated as in Fig. 2a. Cells were lysed to obtain total protein fractions and the ratio of LC3-II to LC3-I protein expression was analyzed by Western blot. Left is the average relative ratio of LC3-II/LC3-I protein expression of each group. Right is a representative image of at least three separate blots showing LC3-I and LC3-II protein expression. ##p < 0.01 versus control; *p < 0.05 versus CoCl2 alone

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