Fig. 2

PKCε expression was gradually reduced in the hippocampus after tGCI. a The mouse hippocampus was isolated at different time points after 10 min of tGCI. Hippocampi of mice without tGCI were used as controls (con). Cytosolic fractions of hippocampus were analyzed by Western blotting using antibodies against PKCε. β-Actin was used as a loading control. b PKCε and β-Actin protein bands were quantified by densitometry (n = 4–7). PKCε immunoreactivity normalized to β-Actin (PKCε/β-Actin) was compared between means using one-way ANOVA with Newman–Keuls post hoc tests. There were significant reductions of PKCε/β-Actin at 4 (*P < 0.05), 24 (**P < 0.005), 48 and 72 h (***P < 0.0005) after tGCI as compared to controls