Fig. 4

Schematics of the data interpretation. a In control conditions local Ca²⁺ accumulation in the close proximity to intracellular domains of activated NMDARs is prevented by NCX-transported Ca²⁺ extrusion. The integral Ca²⁺ entry into neurons via activated NMDARs is large because of moderate Ca²⁺-dependent NMDAR desensitization (CDD). This causes prominent cytozolic [Ca²⁺]_i increase (↑↑↑Ca ²⁺_i ). Typical whole-cell current (I_NMDA) and fluorescent Ca²⁺ probe response are indicated below the panel. b The destruction of lipid rafts increases a distance between NMDARs and NCXs which prevents fast removal of Ca²⁺ entering via open NMDAR channels. Local Ca²⁺ accumulation in the close proximity of intracellular domains of activated NMDAR enhances their CDD and limits NMDAR current steady-state amplitude and as a consequence weakens total cytosolic Ca²⁺ accumulation. Typical whole-cell current (I_NMDA) and fluorescent Ca²⁺ probe response ([Ca²⁺]_i) are indicated below the panel. For further explanation, see "Discussion" section