Fig. 5

Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 DRG. Positive for GlyRα3 are shown in green (a), positive for Gephyrin are shown in red (b) and positive for NeuN are shown in blue (c). Double-labelled images of GlyRα3 and Gephyrin (d), GlyRα3 and NeuN (e) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons of DRG. IF experiments from three different sham controls were performed. Secondary Abs only were included as negative controls. Scale bars = 20 µm