Fig. 7
Group ventral to the GNG (vGNG). a Honeybee brain scheme focusing on the posterior brain. AmFoxP neurons are indicated with white circles within the boxed area which indicates the localization of panels (b–l). b Confocal image (60 µm confocal stack) of a forager brain backfilled with lucifer yellow (green) and subsequently stained with the AmFoxP42kDa antiserum (white label). The large green-labeled neuron lying on the brain midline is a VUMmx neuron. c–l dISH stainings show AmFoxP transcript (magenta), neurotransmitter transcript (cyan) and DAPI-stained nuclei (blue) on 12 µm cryosections. White arrowheads point to neurons with co-localized probe signal. c Cryosections of a 4 days old individual from the ‘honeycomb’ group. d, e Adjacent cryosections of a 15 days old individual from the ‘honeycomb’ group. f Cryosections of a 15 days old individual from the ‘incubator’ group. g, h Adjacent cryosections of a 27 days old individual from the ‘incubator’ group. i–l Adjacent cryosections of a 50 days old individual from the ‘control’ group. For explanations of pictograms see Table 1, for anatomical abbreviations see Table 5. Scale bar b: 100 µm, as orientation for all other panels