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Fig. 7 | BMC Neuroscience

Fig. 7

From: Identification of the neurotransmitter profile of AmFoxP expressing neurons in the honeybee brain using double-label in situ hybridization

Fig. 7

Group ventral to the GNG (vGNG). a Honeybee brain scheme focusing on the posterior brain. AmFoxP neurons are indicated with white circles within the boxed area which indicates the localization of panels (bl). b Confocal image (60 µm confocal stack) of a forager brain backfilled with lucifer yellow (green) and subsequently stained with the AmFoxP42kDa antiserum (white label). The large green-labeled neuron lying on the brain midline is a VUMmx neuron. cl dISH stainings show AmFoxP transcript (magenta), neurotransmitter transcript (cyan) and DAPI-stained nuclei (blue) on 12 µm cryosections. White arrowheads point to neurons with co-localized probe signal. c Cryosections of a 4 days old individual from the ‘honeycomb’ group. d, e Adjacent cryosections of a 15 days old individual from the ‘honeycomb’ group. f Cryosections of a 15 days old individual from the ‘incubator’ group. g, h Adjacent cryosections of a 27 days old individual from the ‘incubator’ group. il Adjacent cryosections of a 50 days old individual from the ‘control’ group. For explanations of pictograms see Table 1, for anatomical abbreviations see Table 5. Scale bar b: 100 µm, as orientation for all other panels

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