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Fig. 5 | BMC Neuroscience

Fig. 5

From: Identification of the neurotransmitter profile of AmFoxP expressing neurons in the honeybee brain using double-label in situ hybridization

Fig. 5

Cluster ‘lateral to the lateral horn’ (lLH). a Honeybee brain schematic drawing focuses on the LH and ME. AmFoxP neurons are indicated with small empty circles. Rectangle indicates the localization of panels (co). b Confocal image (2 µm confocal section) of a forager brain stained with the AmFoxP42kDa antiserum (white label). co dISH stainings showing AmFoxP transcript (magenta), neurotransmitter transcript (cyan) and DAPI-stained nuclei (blue) on 12 µm brain cryosections. Small rectangular insets in c, g, ko show co-localization of the two probes. Larger rectangular insets in b, c show densely-packed small nuclei of the lLH, stained with AmFoxP42kDa antiserum (b) and DAPI (c). cf Adjacent cryosections of a 15 days old individual from the ‘unmanipulated’ group. gj Adjacent cryosections of a 31 days old individual from the ‘incubator’ group. k Newly emerged individual. l 11 days old individual from the ‘honeycomb’ group. m 15 days old individual from the ‘incubator’ group. n 27 days old individual of the ‘incubator’ group. o Cryosection of a 50 days old individual from the ‘unmanipulated’ group. For explanations of pictograms see Table 1, for anatomical abbreviations see Table 5. Scale bar b, c: 100 µm, as orientation for all other panels

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