Fig. 3

Group ‘around (peri) antennal lobe’ (pAL) and cluster ‘anteriodorsal to the esophageal hole’ (adES). a Schematic drawing of the honeybee AL. AmFoxP neurons are depicted as empty circles, arrows point to the two neuron populations. b Confocal image (2 µm confocal section) of a pupal brain stained with the AmFoxP^42kDa antiserum (white label). c–o dISH stainings show AmFoxP transcript (magenta), neurotransmitter marker transcript (cyan) and DAPI-stained nuclei (blue). Large rectangular insets show higher magnifications of small boxed regions, with white label reflecting co-expressing neurons. Some double-labeled neurons are also visible in circled areas in c, j, m. c–f Adjacent cryosections of a 15 days old individual from the ‘unmanipulated’ condition. g–i Adjacent cryosections of a 15 days old individual from the ‘honeycomb’ condition, anterior (g) and posterior (h, i). j–o Adjacent cryosections of a 31 days old individual from the ‘incubator’ condition, anterior (j–m) and posterior (n, o). Pictograms refer to the conditions outlined in Table 1. Anatomical abbreviations see Table 5. Scale bar b: 100 µm, as orientation for all other panels