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Fig. 14 | BMC Neuroscience

Fig. 14

From: Identification of the neurotransmitter profile of AmFoxP expressing neurons in the honeybee brain using double-label in situ hybridization

Fig. 14

AmFoxP and AmVGluT mRNA expression in the KC. aj ISH stainings on 12 µm cryosections of individuals taken from the different treatment groups (symbols, Table 1) show AmVAchT (ae) and AmVGluT (fj) expression in different KC subpopulations. AmVAchT was restricted primarily, in workers aged younger than 5 days, to the sKC and mKC (a, b). With increasing age, the lKC also started expressing AmVAchT (ce). Simultaneously, the expression intensity in the sKC decreased until, in a very old forager (50 days) there was no more difference between the sKC/mKC and the lKC detectable (e). fj Very low AmVGluT signals in the sKC/mKC were detected in almost half of the samples (all shown here). Age and treatment groups are indicated with symbols that can be seen Table 1. k, l Two different forager brains (100 µm sections) before (k) and after (l) calyx dissection for RT-qPCR experiments (nuclear staining with SYTOX green). Scale bars: 100 µm, in a, f as orientation for the panels (be) and (gj). m Relative expression levels of vesicular transporters in calyx extracts, measured by RT-qPCR. In adult foragers AmVAchT levels were ten times higher than AmVGluT levels (paired Student’s t(8) = 13.1, p < .0001). AmIAAT and AmVMAT levels were 7–11 times lower than AmVGluT levels (AmVIAAT: paired Student’s t(8) = 30, p < .0001, AmVMAT: paired Student’s t(8) = 27.4, p < .0001)

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