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Fig. 12 | BMC Neuroscience

Fig. 12

From: Identification of the neurotransmitter profile of AmFoxP expressing neurons in the honeybee brain using double-label in situ hybridization

Fig. 12

AmFoxP expressing Kenyon cells (KC) of the mushroom bodies. a Single confocal sections (1 µm) show the KC of a whole-mount pupal brain stained with the AmFoxP42kDa antiserum (white label), (aI) anterior, (aII) posterior. The calyx is encircled with a dashed line. White arrowheads point to the elongated AmFoxP neuron clusters (mKC). be ISH stainings show AmFoxP transcript (magenta) and AmVAchT transcript (cyan) on 12 µm cryosections of individuals taken from the different treatment groups. Age and treatment group are indicated with symbols that can be seen in Table 1. Magnifications of inset in b is shown in panels (bIII–V). The two transcripts of AmFoxP and AmVAchT are mostly co-expressed in the same cells. f, g dISH stainings show expression of the D2-like dopamine-receptor Amdop3 (cyan) and AmFoxP (magenta). The Amdop3 sense probe (control) did not show any (unspecific) staining (f). Amdop3 is mostly expressed in the lKC but there was a small overlap with the AmFoxP-expressing mKC (gIgIII). Inset in gI is shown magnified in gII. gIII shows a schematic of gI. Blue: nuclear staining (DAPI). Sizes of scale bars are indicated

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