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Fig. 6 | BMC Neuroscience

Fig. 6

From: Expression of alternatively spliced variants of the Dclk1 gene is regulated by psychotropic drugs

Fig. 6

Validation of mianserin-induced changes in Dclk1 expression. Changes in mRNA levels were measured 2 h after administration of mianserin or saline. qPCR analyses were performed using samples from an independent biological experiment (n = 8). TaqMan probes distinguished the following specific transcriptional variants: qPCR “probe 1” spans the exon junction of exons 4 and 5 of the Dclk1 (ENSMUST00000054237) transcript, qPCR “probe 2” spans exons 6 and 7 in Dcl (ENSMUST00000167204), qPCR “probe 3” spans 5′ part of intron 6 of Dclk1 (ENSMUST00000054237), and qPCR “probe 4” spans 3′ part of intron 6 of Dclk1 (ENSMUST00000054237), qPCR “probe 5” spans exons 13 and 14 of Cpg16 (ENSMUST00000198437). The locations of TaqMan probes used for qPCR are labeled (probes 1–5). Bars indicate the S.E.M, ***P < 0.001, one-way ANOVA of the drug factor

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