Fig. 8

Immunohistochemical detection of PV neurons and WFA-positive PNNs in specific regions of juvenile–stressed and control groups. Representative double immunofluorescence images of the CA1, DG (a, a′, b, b′), dAC (c, c′, d, d′), PL (e, e′, f, f′), IL (g, g′, h, h′), and M1 (i, i′, j, j′) are shown. PV neurons are indicated by green fluorescence (Alexa Fluor 488), and WFA-positive PNNs are indicated by red fluorescence (Alexa Fluor 594). Representative double immunofluorescence images of the CA1 (k, k′, l, l′), dAC L2/3 (m, m′, n, n′), dAC L5/6 (o, o′, p, p′), and M1 L2/3 (q, q′, r, r′) are shown at high magnification. Double confocal images of PV and WFA reactivity in control (a, a′, c, c′, e, e′, g, g′, i, i′, k, k′, m, m′, o, o′, q, q′) and stress mice (b, b′, d, d′, f, f′, h, h′, j, j′, l, l′, n, n′, p, p′, r, r′) are shown. Scale bar = 100 μm in j′ (applies to a–j, a′–j′), and 10 μm in r′ (applies to k–r, k′–r′). PV parvalbumin, WFA Wisteria floribunda agglutinin, PNN perineuronal net, dAC dorsal anterior cingulate cortex, PL prelimbic cortex, IL infralimbic cortex, M1 primary motor cortex